Vol. 2 No. 1 (2025): Journal of Horticulture and Agricultural Sciences (JHAS)
Articles

Effect of Salt Stress (NaCl) on Seed Germination and Vegetative Growth of Hollyhock (Althaea rosea L.)

Muhammad Nawaz Baloch
Department of Horticulture, Sindh Agriculture University Tandojam, Pakistan
Muhammad Khan Baloch
Department of Plant Breeding and Genetics, Sindh Agriculture University Tandojam, Pakistan
Asif Raza Baloch
Department of Plant Breeding and Genetics, Sindh Agriculture University Tandojam, Pakistan
Ali Jaan
Department of Plant Breeding and Genetics, Sindh Agriculture University Tandojam, Pakistan
Qamber Khan
Department of Horticulture, Sindh Agriculture University Tandojam, Pakistan
Nawab Jan Baloch
Department of Plant Breeding and Genetics, Sindh Agriculture University Tandojam, Pakistan

Published 2025-06-27

Keywords

  • Salt stress,,
  • NaCl,
  • seed germination,
  • growth,
  • Holly hock

How to Cite

Baloch, M. N., Baloch, M. K., Baloch, A. R., Ali Jaan, Qamber Khan, & Baloch, N. J. (2025). Effect of Salt Stress (NaCl) on Seed Germination and Vegetative Growth of Hollyhock (Althaea rosea L.). Journal of Horticulture and Agricultural Sciences , 2(1), 91–99. https://doi.org/10.63459/jhas.2025.1.19

Abstract

Salinity is alarming issue to crop production worldwide. Evaluation of crop plants at early growth stages is perquisite to determine tolerance of plants to salt stress. The pot experiment was performed during 2019 to analyze the impact of salt stress (NaCl) on germination and growth attributes of hollyhock (Althaea rosea L.) genotypes. The trial was set up at the nursery of Sindh Agriculture University Tandojam employing completely randomized design (factorial). The seed germination and vegetative growth was evaluated in two genotypes of hollyhock that included Halo Blush and Scarlet Eye. The treatments included S1 = Canal irrigation water (Control), S2 =3.0 dS m-1, S3 =6.0 dS m-1, S4 = 9.0 dS m-1 and S5 = 12.0 dS m-1. The germination and growth of plants were substantially affected by salinity levels. The seeds sown by applying canal water produced vigorous plants with higher germination. Moreover, plants irrigated with canal water revealed maximum foliage production with better shoot and root growth. Additionally minimum electrolyte leakage was also noticed in plants irrigated with canal water. Plants irrigated with water having EC 3 dSm-1 ranked 2nd and considerably showed better germination and for other studied parameters. The salinity at 6, 9 and 12 dS m-1 caused simultaneously undesirable impact on all the investigated traits with the exception to electrolyte leakage of leaf that augmented parallel to the elevated salt stress conditions. In case of genotypes “Halo Blush” performed better for most of the investigated traits as compared to “Scarlet Eye” This indicates that Halo Blush had more tolerance to salinity as compared to Scarlet Eye.

References

  1. Abdul-baki, A.A, & Anderson, J.D. (1970). Viability and leaching of sugars from germinating barley. Crop Science, 10(1):31-34.
  2. Ahmad, P., Jaleel, C. A., Salem, M. A., Nabi, G., & Sharma, S. (2010). Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Critical reviews in biotechnology, 30(3): 161-175.
  3. Ashraf, M., Zafar, R., & Ashraf, M. Y. (2003). Time-course changes in the inorganic and organic components of germinating sunflower achenes under salt (NaCl) stress. Flora-Morphology, Distribution, Functional Ecology of Plants, 198(1): 26-36.
  4. Bajji, M., Kinet, J. M., & Lutts, S. (2002). The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Plant growth regulation, 36: 61-70.
  5. Cachorro, P., Ortiz, A., & Cerdá, A. (1993). Growth, water relations and solute composition of Phaseolus vulgaris L. under saline conditions. Plant science, 95(1): 23-29.
  6. Dasgan, H. Y., Aktas, H., Abak, K., & Cakmak, I. (2002). Determination of screening techniques to salinity tolerance in tomatoes and investigation of genotype responses. Plant Science, 163(4): 695-703.
  7. Dkhil, B.B., Issa, A., &. Denden, M. (2014). Germination and Seedling Emergence of prime okra(Abelmoschus esculentus L.) Seeds under salt stress and low temperature. American Journal of Plant Physiology, 9(2):38-45.
  8. El Sabagh, A., Islam, M. S., Skalicky, M., Ali Raza, M., Singh, K., Anwar Hossain, M., & Arshad, A. (2021). Salinity stress in wheat (Triticum aestivum L.) in the changing climate: Adaptation and management strategies. Frontiers in Agronomy, 3: 661932.
  9. Fahamiya, N., Shiffa, M., & Aslam, M. (2016). A comprehensive review on Althaea rosea Linn. Journal of Pharmaceutical Research, 6(11): 6888-6894.
  10. Guan, B., Zhou, D., Zhang, H., Tian, Y., Japhet, W., & Wang, P. (2009). Germination responses of Medicago ruthenica seeds to salinity, alkalinity, and temperature. Journal of Arid Environments, 73(1): 135-138.
  11. Hayat, K., Bundschuh, J., Jan, F., Menhas, S., Hayat, S., Haq, F., & Zhou, P. (2020). Combating soil salinity with combining saline agriculture and phytomanagement with salt-accumulating plants. Critical Reviews in Environmental Science and Technology, 50(11): 1085-1115.
  12. Ibrahim, E. A. (2016). Seed priming to alleviate salinity stress in germinating seeds. Journal of plant physiology, 192: 38-46.
  13. Jamil, M., D. B. Lee, K.Y. Jung, M. Ashraf, S.C. Lee & Rha, S.E. (2006). Effect of salt (NaCl) stress on germination and early seedling growth of four vegetable species. Journal of Central European Agriculture. 7(2): 273-282.
  14. Kasukabe, Y., He, L., Nada, K., Misawa, S., Ihara, I., & Tachibana, S. (2004). Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana. Plant and Cell Physiology, 45(6): 712-722.
  15. Keutgen, A. J., & Pawelzik, E. (2008). Quality and nutritional value of strawberry fruit under long term salt stress. Food chemistry, 107(4): 1413-1420.
  16. Larsen, S.U. and C. Andreasen. (2004). Light and heavy turf-grass seeds differ in germination percentage and mean germination thermal time. Crop Science. 44:1710-1720.
  17. Munns, R. and M. Tester. (2008). Mechanism of salinity tolerance. Annual Review of Plant Biology. 59: 651-681.
  18. Parvaiz A.L., A. K. Bhardwaj and F. A. Bahar. (2015). Study of indigenous/traditional medicinal plant knowledge anendeavour towards new drug discovery. African Journal of Traditional, Complementary & Alternative Medicines. 12(2): 73-95.
  19. Rasha, S. El-S and A. A El-Sheshtawy. (2017). Improving seed germination of Althaea rosea L. under salt stress by seed soaking with silicon and nano silicon. Egyptian Journal of Plant Breeding. 21 (5): 764 –777
  20. Sayyed, A., Gul, H., Zia Ullah, & Hamayun, M. (2014). Effect of salt stress on growth of Tagetes erecta L. Pakhtunkhwa Journal of Life Sciences, 2(34): 96–106.
  21. Shalata, A., & Neumann, P. M. (2001). Exogenous ascorbic acid (vitamin C) increases resistance to salt stress and reduces lipid peroxidation. Journal of experimental botany, 52(364): 2207-2211.
  22. Shelke, D., Pandey, M., Nikalje, G., Zaware, B., Suprasanna, P., & Nikam, T. (2017). Salt-responsive physiological, photosynthetic and biochemical attributes at early seedling stage for screening soybean genotypes. Plant Physiology and Biochemistry, 118: 519–528.
  23. Sikder, R. K., Wang, X., Jin, D., Zhang, H., Gui, H., Dong, Q., Pang, N., Zhang, X., & Song, M. (2020). Screening and evaluation of reliable traits of upland cotton (Gossypium hirsutum L.) genotypes for salt tolerance at the seedling growth stage. Journal of Cotton Research, 3(1): 11–23.
  24. Silveira, E. A., Kac, G., & Barbosa, L. S. (2009). Prevalência e fatores associados à obesidade em idosos residentes em Pelotas, Rio Grande do Sul, Brasil: classificação da obesidade segundo dois pontos de corte do índice de massa corporal. Cadernos de Saúde Pública, 25(7): 1569-1577.
  25. Singh, A. (2022). Soil salinity: A global threat to sustainable development. Soil Use and Management, 38(1): 39-67.
  26. Statistix, (2006). Statistix 8 user guide, version 1.0. Analytical software, P.O. Box 12185, Tallahassee fl 32317 USA. Copyright @ 2006 by Analytical Software.
  27. Tobe, K., Zhang, L., & Omasa, K. (2003). Alleviatory effects of calcium on the toxicity of sodium, potassium and magnesium chlorides to seed germination in three non-halophytes. Seed Science Research, 13(1):47-54.
  28. Werner, J. E., & Finkelstein, R. R. (1995). Arabidopsis mutants with reduced response to NaCl and osmotic stress. Physiologia Plantarum, 93(4): 659-666.
  29. Wu, C., Wang, Z., Song, X., Feng, X. S., Abnet, C. C., He, J. & Chanock, S. J. (2014). Joint analysis of three genome-wide association studies of esophageal squamous cell carcinoma in Chinese populations. Nature genetics, 46(9): 1001-1006.
  30. Yadav, S., Irfan, M., Ahmad, A., & Hayat, S. (2011). Causes of salinity and plant manifestations to salt stress: A review. Journal of Environmental Biology, 32(5): 667–685.